Application of Near Infrared Reflectance Spectroscopy (NIRS) for Macronutrients Analysis in Alfalfa (\u3ci\u3eMedicago sativa\u3c/i\u3e L.)

Near infrared reflectance spectroscopy was used to assess the mineral composition of alfalfa (Medicago sativa L.) as a tool for nutritional diagnosis. One hundred and ninety four (n = 194) samples of alfalfa from different locations representing a wide range of soils were used. Samples were reflectance scanned in a NIRS 6500 (NIRSystems, USA) instrument. The coefficients of determination (R2) of the regression estimate of the concentration of nitrogen, calcium, phosphorus, potassium, magnesium and sulphur and the errors in cross validation (SECV) were 0.93 (SECV: 1.6), 0.95 (SECV: 1.3), 0.93 (SECV: 1.9), 0.88 (SECV: 2.8), 0.82 (SECV: 1.9) and 0.75 (SECV: 4.7) respectively. The best NIRS predictions were obtained for calcium and nitrogen, meanwhile the poorest was obtained for sulphur

Chloroplast DNA Inheritance in the Orchid Anacamptis palustris Using Single-Seed Polymerase Chain Reaction

The modality of chloroplast inheritance in orchids has been investigated only in a few species due to the difficulties associated with the analysis of large progeny numbers from experimental crosses. To test chloroplast DNA inheritance in the orchid Anacamptis palustris, we took advantage of the presence of a highly variable minisatellite repeat located in the tRNALEU intron in the chloroplast genome. Seed progeny obtained from experimental crosses between parental individuals carrying different chloroplast DNA (cpDNA) minisatellite repeat numbers were analyzed using a single-seed polymerase chain reaction (PCR) protocol. All examined seeds displayed the maternal cpDNA haplotypes, indicating that cpDNA inheritance is strictly maternal in this Mediterranean orchid species. No evidence for paternal leakage was found. This finding concurs with results obtained from PCR amplifications of pollen massulae that exclude the presence of chloroplast DNA in the pollen tetrad

An epistatic mini-circuitry between the transcription factors Snail and HNF4a controls liver stem cell and hepatocyte features exhorting opposite regulation on stemness-inhibiting microRNAs

Preservation of the epithelial state involves the stable repression of EMT program while maintenance of the stem compartment requires the inhibition of differentiation processes. A simple and direct molecular mini-circuitry between master elements of these biological processes, may provide the best device to keep balanced such complex phenomena. In this work, we show that in hepatic stem cell Snail, a transcriptional repressor of the hepatocyte differentiation master gene HNF4, directly represses the expression of the epithelial microRNAs-200c and -34a, which in turn target several stem cell genes. Notably, in differentiated hepatocytes HNF4, previously identified as a transcriptional repressor of Snail, induces the microRNAs-34a and -200a, b, c that, when silenced, causes epithelial dedifferentiation and reacquisition of stem traits. Altogether these data unveiled Snail, HNF4 and microRNAs -200a, b, c and -34a as epistatic elements controlling hepatic stem cell maintenance/differentiation

Interpreting and Reporting Principal Component Analysis in Food Science Analysis and Beyond

Principal component analysis (PCA) is one of the most widely used data mining techniques in sciences and applied to a wide type of datasets (e.g. sensory, instrumental methods, chemical data). However, several questions and doubts on how to interpret and report the results are still asked every day from students and researchers. This brief communication is inspired in relation to those questions asked by colleagues and students. Please note that this article is a focus on the practical aspects, use and interpretation of the PCA to analyse multiple or varied data sets. In summary, the application of the PCA provides with two main elements, namely the scores and loadings. The scores provide with a location of the sample where the loadings indicate which variables are the most important to explain the trends in the grouping of samples

In situ polymerization of soil organic matter by oxidative biomimetic catalysis.

Background: Agricultural practices that enhance organic matter content in soil can play a central role in sequestering soil organic carbon (SOC) and reducing greenhouse gases emissions. Methods: We used a water-soluble iron-porphyrin to catalyze directly in situ oxidative polymerization of soil organic matter in the presence of H2O2 oxidant, with the aim to enhance OC stabilization, and, consequently, reduce CO2 emissions from soil. The occurred SOC stabilization was assessed by monitoring soil aggregate stability, OC distribution in water-soluble aggregates, soil respiration, and extraction yields of humic and fulvic acids. Results: Soil treatment with H2O2 and iron-porphyrin increased the physical stability of water-stable soil aggregates and the total OC content in small aggregates, thereby suggesting that the catalyzed oxidative polymerization increased OC in soil and induced a soil physical improvement. The significant reduction of CO2 respired by the catalyst- and H2O2-treated soil indicated an enhanced resistance of polymerized SOC to microbial mineralization. The catalyzed oxidative polymerization of SOC also significantly decreased the extraction yields of humic and fulvic acids from soil. Conclusions: The oxidative catalytic technology described here may become an efficient agricultural practice for OC sequestration in soils and contribute to mitigate global changes

The Role of Lichens, Mosses, and Vascular Plants in the Biodeterioration of Historic Buildings: A Review

Biodeterioration is defined as the alteration of a given substrate due to a combination of physical and chemical factors produced by living organisms when attached to such materials. This phenomenon attracts scientific research attention due to its risk in causing destruction to outdoor cultural rock heritage sites. In this review, an update on the state-of-art regarding the biodeterioration phenomenon is represented in order to highlight the type of colonizing vegetation and possible mechanisms behind the corresponding deterioration. For this reason, 62 articles with a focus on lichens, mosses, and higher plants were investigated by evaluating the role of construction materials and different plant species related to the hazard index. The results showed that trees and shrubs are the most harmful plant life forms, for example, Ficus carica, Ailanthus altissima, and Capparis spinosa, while regarding building materials, those characterized by high porosity, such as andesite and argillaceous limestone, are more vulnerable to plant colonization. Further studies are needed to examine in detail the relationship between colonizing organisms, intrinsic elements of the substrate, and external factors, as well as the refinement of measures to prevent and control colonization by plants

Climatic and anthropogenic factors affect Ailanthus altissima invasion in a Mediterranean region

Ailanthus altissima is an aggressive invasive tree worldwide, but the ecological factors that lead to the spread of this species in Mediterranean ecosystems are still unclear. Here we aim to identify such factors, focusing on the interaction of human activity with climatic conditions. We determined the occurrence and abundance of Ailanthus in 240 sites and studied their relationship with 20 variables representing climatic, geographic, and topographic factors, as well as land use, in the region of Campania (southern Italy). Overall, we found that temperature and rainfall in Campania are suitable for Ailanthus, with the only major constraint being the temperature at an altitude exceeding 900 m a.s.l. We found that Ailanthus is unable to spread where the mean annual temperature is lower than 11.1 °C. By contrast, precipitation variables showed poor correlation with Ailanthus distribution, suggesting that rainfall in the selected study sites is suitable to sustain the growth of this tree. About land use variables, roads were the primary landscape feature along which this species spread and invaded new areas. Roads probably combine high propagule pressure and favorable growing conditions in terms of available resources i.e., light, water, and mineral nutrients, that allow Ailanthus to establish and spread along roadside edges in different ecosystems. In conclusion, we found that climate and human-associated variables are correlated with the current occurrence of Ailanthus, with the temperature being more influential at high elevation sites and road distance playing a prominent role in low elevation areas

Molecular characteristics of humic substances from different origins and their effects on growth and metabolism of Pinus laricio callus

Abstract Humic susbtances (HS) are increasingly applied as biostimulants in agriculture, though their mechanism of action is not yet completely understood due to their complex and heterogeneous composition. Here, we isolated thirteen different humic and fulvic acids from different sources, such as soils, composts, peat, leonardite and lignite, and characterized the distribution of their carbon components by 13C-CPMAS–NMR spectroscopy. Callus of Pinus Laricio, as a model plant species, was treated with different humic extracts and its growth and content of carbohydrates, phenols, and enzyme related to the nitrogen metabolism (invertase, glutamine synthetase, glutamate synthase, phosphoenoly pyruvate carboxylase, malate dehydrogenase) and stress resistance (catalase) were monitored. While a multivariate statistical analysis of NMR results well-separated the HS characteristics based on their origin, humic materials generally increased callus growth, as expected, with largest effects being exerted by the mostly polar humic acids from composts. However, the rest of measured parameters were not linearly related to hydrophobicity and aromaticity of humic isolates as well as their origin, but their conformational dynamics had to be advocated to explain their effects on callus cellular components. This work confirms that HS of various origin can act as sustainable biostimulants of plant growth, though the comprehension of their effects on plants biochemical activities requires further research and additional understanding of their molecular composition and conformational behaviour. Graphical Abstrac

Multistep, sequential control of the trafficking and function of the multiple sulfatase deficiency gene product, SUMF1 by PDI, ERGIC-53 and ERp44.

Sulfatase modifying factor 1 (SUMF1) encodes for the formylglicine generating enzyme, which activates sulfatases by modifying a key cysteine residue within their catalytic domains. SUMF1 is mutated in patients affected by multiple sulfatase deficiency, a rare recessive disorder in which all sulfatase activities are impaired. Despite the absence of canonical retention/retrieval signals, SUMF1 is largely retained in the endoplasmic reticulum (ER), where it exerts its enzymatic activity on nascent sulfatases. Part of SUMF1 is secreted and paracrinally taken up by distant cells. Here we show that SUMF1 interacts with protein disulfide isomerase (PDI) and ERp44, two thioredoxin family members residing in the early secretory pathway, and with ERGIC-53, a lectin that shuttles between the ER and the Golgi. Functional assays reveal that these interactions are crucial for controlling SUMF1 traffic and function. PDI couples SUMF1 retention and activation in the ER. ERGIC-53 and ERp44 act downstream, favoring SUMF1 export from and retrieval to the ER, respectively. Silencing ERGIC-53 causes proteasomal degradation of SUMF1, while down-regulating ERp44 promotes its secretion. When over-expressed, each of three interactors favors intracellular accumulation. Our results reveal a multistep control of SUMF1 trafficking, with sequential interactions dynamically determining ER localization, activity and secretion